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blot nitro stripping buffer  (LI-COR)


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    Structured Review

    LI-COR blot nitro stripping buffer
    Blot Nitro Stripping Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blot+nitro+stripping+buffer/pmc06536551-438-21-25
    Average 86 stars, based on 1 article reviews
    blot nitro stripping buffer - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Stripping Membranes:

    Article Title: Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein–Gremlin signaling pathways
    Article Snippet: Western blots were imaged on a LICOR Odyssey CLx using infrared fluorescent detection. .. After probing with antibodies against SMAD4, SLIT2, and phospho-SMAD1/5/9, the blots were stripped with New Blot Nitro Stripping Buffer (LICOR, 928-40030) and reprobed with loading control antibodies. .. To quantify SMAD4 and SLIT2 expression, α/β Tubulin was used as a loading control.

    Article Title: The viral protein corona directs viral pathogenesis and amyloid aggregation
    Article Snippet: The gels were then transferred onto nitrocellulose membranes using the IBlot® system (Invitrogen) and, subsequently, the membranes were blocked with Odyssey Blocking Buffer (LI-COR Biosciences GmbH) for 1.5 h. Membranes were incubated overnight at 4 °C with anti-SP-A antibody [6F10] (ab51891, Abcam) diluted at 1:500, then probed the next day using goat anti-human IgG (IRDye 800, red, LI-COR Biosciences) and goat anti-mouse IgG (IRDye 680, green, LI-COR Biosciences). .. To normalize the bands to a loading control, scanned membranes were stripped for 10 min at 65 °C using 1× New Blot Nitro Stripping buffer (LI-COR Biosciences), blocked for 1.5 h, and then incubated with anti-RSV antibody (ab20745, Abcam) at 1:500 dilution for 2 h at RT before probing with secondary antibody (donkey anti-goat, IRDye 680, green, LI-COR Biosciences). .. All western blotting signals were scanned using the Odyssey Imager (LI-COR Biosciences GmbH) and quantification was performed on the images using ImageJ software.

    Article Title: Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein–Gremlin signaling pathways
    Article Snippet: Western blottings were imaged on a LICOR Odyssey CLx using infrared fluorescent detection. .. After probing with antibodies against SMAD4, SLIT2, and phospho-SMAD1/5/9, the blots were stripped with New Blot Nitro Stripping Buffer (LICOR, 928-40030) and reprobed with loading control antibodies. .. To quantify SMAD4 and SLIT2 expression, α/β tubulin was used as a loading control.

    Article Title: SLC25 Family Member Genetic Interactions Identify a Role for HEM25 in Yeast Electron Transport Chain Stability
    Article Snippet: Excess antibody was washed off with 0.1% Tween-20 in PBS and proteins were detected and imaged by using a LI-COR Odyssey Infrared Imaging System. .. The membranes were stripped with New Blot Nitro Stripping Buffer (LI-COR) for 10 min under constant shaking at room temperature, and reprobed with anti-porin antisera ( Por1 ) as a loading control. ..

    Article Title: Reactivation of cocaine reward memory engages the Akt/GSK3/mTOR signaling pathway and can be disrupted by GSK3 inhibition.
    Article Snippet: .. Membranes were stripped of antibodies using the New Blot nitro stripping buffer (LI-COR) and re-probed with anti-β-tubulin (1: 400,000; Sigma-Aldrich, St. Louis, MO) to control for potential differences in protein loading and transfer. ..

    Article Title: NK-3 receptor antagonism prevents behavioral sensitization to cocaine: A role of glycogen synthase kinase-3 in the nucleus accumbens
    Article Snippet: .. Membranes were stripped of antibodies using the New Blot nitro stripping buffer (LI-COR) and re-probed with anti-α-tubulin [1:80,000 (Sigma-Aldrich)] to control for differences in protein loading and during protein transfer. ..

    Control:

    Article Title: Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein–Gremlin signaling pathways
    Article Snippet: Western blots were imaged on a LICOR Odyssey CLx using infrared fluorescent detection. .. After probing with antibodies against SMAD4, SLIT2, and phospho-SMAD1/5/9, the blots were stripped with New Blot Nitro Stripping Buffer (LICOR, 928-40030) and reprobed with loading control antibodies. .. To quantify SMAD4 and SLIT2 expression, α/β Tubulin was used as a loading control.

    Article Title: The viral protein corona directs viral pathogenesis and amyloid aggregation
    Article Snippet: The gels were then transferred onto nitrocellulose membranes using the IBlot® system (Invitrogen) and, subsequently, the membranes were blocked with Odyssey Blocking Buffer (LI-COR Biosciences GmbH) for 1.5 h. Membranes were incubated overnight at 4 °C with anti-SP-A antibody [6F10] (ab51891, Abcam) diluted at 1:500, then probed the next day using goat anti-human IgG (IRDye 800, red, LI-COR Biosciences) and goat anti-mouse IgG (IRDye 680, green, LI-COR Biosciences). .. To normalize the bands to a loading control, scanned membranes were stripped for 10 min at 65 °C using 1× New Blot Nitro Stripping buffer (LI-COR Biosciences), blocked for 1.5 h, and then incubated with anti-RSV antibody (ab20745, Abcam) at 1:500 dilution for 2 h at RT before probing with secondary antibody (donkey anti-goat, IRDye 680, green, LI-COR Biosciences). .. All western blotting signals were scanned using the Odyssey Imager (LI-COR Biosciences GmbH) and quantification was performed on the images using ImageJ software.

    Article Title: Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein–Gremlin signaling pathways
    Article Snippet: Western blottings were imaged on a LICOR Odyssey CLx using infrared fluorescent detection. .. After probing with antibodies against SMAD4, SLIT2, and phospho-SMAD1/5/9, the blots were stripped with New Blot Nitro Stripping Buffer (LICOR, 928-40030) and reprobed with loading control antibodies. .. To quantify SMAD4 and SLIT2 expression, α/β tubulin was used as a loading control.

    Article Title: SLC25 Family Member Genetic Interactions Identify a Role for HEM25 in Yeast Electron Transport Chain Stability
    Article Snippet: Excess antibody was washed off with 0.1% Tween-20 in PBS and proteins were detected and imaged by using a LI-COR Odyssey Infrared Imaging System. .. The membranes were stripped with New Blot Nitro Stripping Buffer (LI-COR) for 10 min under constant shaking at room temperature, and reprobed with anti-porin antisera ( Por1 ) as a loading control. ..

    Article Title: Reactivation of cocaine reward memory engages the Akt/GSK3/mTOR signaling pathway and can be disrupted by GSK3 inhibition.
    Article Snippet: .. Membranes were stripped of antibodies using the New Blot nitro stripping buffer (LI-COR) and re-probed with anti-β-tubulin (1: 400,000; Sigma-Aldrich, St. Louis, MO) to control for potential differences in protein loading and transfer. ..

    Article Title: NK-3 receptor antagonism prevents behavioral sensitization to cocaine: A role of glycogen synthase kinase-3 in the nucleus accumbens
    Article Snippet: .. Membranes were stripped of antibodies using the New Blot nitro stripping buffer (LI-COR) and re-probed with anti-α-tubulin [1:80,000 (Sigma-Aldrich)] to control for differences in protein loading and during protein transfer. ..

    Incubation:

    Article Title: The viral protein corona directs viral pathogenesis and amyloid aggregation
    Article Snippet: The gels were then transferred onto nitrocellulose membranes using the IBlot® system (Invitrogen) and, subsequently, the membranes were blocked with Odyssey Blocking Buffer (LI-COR Biosciences GmbH) for 1.5 h. Membranes were incubated overnight at 4 °C with anti-SP-A antibody [6F10] (ab51891, Abcam) diluted at 1:500, then probed the next day using goat anti-human IgG (IRDye 800, red, LI-COR Biosciences) and goat anti-mouse IgG (IRDye 680, green, LI-COR Biosciences). .. To normalize the bands to a loading control, scanned membranes were stripped for 10 min at 65 °C using 1× New Blot Nitro Stripping buffer (LI-COR Biosciences), blocked for 1.5 h, and then incubated with anti-RSV antibody (ab20745, Abcam) at 1:500 dilution for 2 h at RT before probing with secondary antibody (donkey anti-goat, IRDye 680, green, LI-COR Biosciences). .. All western blotting signals were scanned using the Odyssey Imager (LI-COR Biosciences GmbH) and quantification was performed on the images using ImageJ software.



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